CET Karnataka Engineering CET - Karnataka Engineering Solved Paper-2001

  • question_answer
    The test used for identifying peptide linkage in proteins is:

    A)  Borsche's test  

    B)  Molisch's test

    C)  Ninhydrin test

    D)  Biuret test

    Correct Answer: D

    Solution :

    For the identification of peptide linkage the test performed is Biuret test. \[N{{H}_{2}}CON{{H}_{2}}\xrightarrow{\text{Heat}}N{{H}_{3}}+NH=C=O\] \[\underset{\text{Peptide}\,\text{bond}}{\mathop{NH=C=O\,+}}\,\,N{{H}_{2}}CON{{H}_{2}}\xrightarrow{{}}\] \[\underset{\text{Biuret}}{\mathop{{{H}_{2}}NCONHCON{{H}_{2}}}}\,\]


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