Electrophoresis
Category : 11th Class
It is an another technique of separation. In which patricles of different charges and sizes are separated under the influence of electric field. e.g., nucleic acids, proteins, amino acids, nucleotides can be separated by this method. The technique was discovered by Russian physicist Alexender Reuss in 1807.
In immunoelectrophoresis antibodies coupled with radioisotopes, specific enzymes or fluorescent dyes are used in detection of particular proteins. The technique is highly sensitive. It can separate molecules in picogram and nanogram quantities and distinguish proteins which differ from each other in only one amino acid.
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