(c) If any protein encoding gene is
expressed in a heterologous host, it is called are combinant protein. The cells
harbouring cloned genes of interest may be grown on a small scale in the
The cultures may be used for extracting
the desired protein and then purifying it by using different separation
The cells can also be multiplied
in a continuous culture system where in the used medium is drained out from one
side while fresh medium is added from the other to maintain the cells in their
physiologically most active log/exponential phase. This type of culturing
method produces a larger biomass leading to higher yields of desired protein.
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