question_answer 28)

  A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be (a) human gene may have intron which bacteria cannot process (b) amino acid codons for humans and bacteria are different (c) human protein is formed but degraded by bacteria (d) All of the above

Answer:

                  (a) The process of making recombinant DNA molecule involves the introduction of a desired gene into the DNA of a host that will produce the desired protein. Inducing a cloned eukaryotic gene to function in a prokaryotic host can be difficult sometime. The presence of long non-coding introns in eukaryotic genes may prevent correct expression of these genes in prokaryoteswhich lack RNA-splicing machinery.