(a) The process of making
recombinant DNA molecule involves the introduction of a desired gene into the
DNA of a host that will produce the desired protein. Inducing a cloned
eukaryotic gene to function in a prokaryotic host can be difficult sometime.
The presence of long non-coding introns in eukaryotic genes may prevent correct
expression of these genes in prokaryoteswhich lack RNA-splicing machinery.
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