(a) All of the given features are
important to facilitate cloning into a vector but out of them origin of
replication (ori) is the most important one.
This is due to the following reasons
(i) Ori is a DNA sequence that
is responsible for initiating replication. Any piece of DNA when linked to this
sequence can replicate within the host cells.
(ii) Ori also controls the copy
numbers of the linked DNA.
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