A) Non-recombinant bacteria containing \[\beta \]galactosidase
B) Insertional inactivation ofoc-galactosidase in non-recombinant bacteria
C) Insertional inactivation ofa-galactosidase in recombinant bacteria
D) Inactivation of glycosidase enzyme in recombinant bacteria
Correct Answer: C
Solution :
The colonies of recombinant bacteria appear white in contrast to blue calories of non-recombinant bacteria because of insertional inactivation of alpha galactosidase in recombinant bacteria. Alpha galactosidase is a glycoside hydrolase enzyme that hydrolyse the terminal alpha galactosyl moieties from glycolipids and glycoprotein. It is encoded by the GLA gene. \[\beta -\]galactosidase is an exoglycosidase, which hydrolyzes the \[\beta -\]glycosidic bond formed between a galactose and its organic rhoiety.
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