A) demonstrate DNA as genetic material
B) replicate specific DNA sequences at high temperatures
C) determine minerals in biological tissue
D) replicate RNA sequences at low temperatures
Correct Answer: B
Solution :
Polymerase Chain Reaction (PCR) is a technique used to replicate a fragment of DNA so as to produce many copies of a particular DNA sequence. In this technique, two strands of the DNA are separated by heating and short sequences of a single DNA strand (primers) are added, together with a supply of free nucleotides and DNA polymerase obtained from a bacterium that can withstand extreme heat. In a series of heating and cooling cycles, the DNA sequence flanked by the primers doubles with each cycle and is thus rapidly amplified.
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