EAMCET Medical EAMCET Medical Solved Paper-2006

  • question_answer
    The enzyme employed for amplification of DNA during PCR is commercially obtained from :

    A)  Streptococcus pyogenes

    B)  Bacillus licheniformis

    C)  Trichonderma reesi

    D)  Thermos aquaticus

    Correct Answer: D

    Solution :

    Heat stable DNA polymerase .i.e., Taq (isolated from Thermus acquaticus), Pfu (from Pyrococcus furiosus) and vent (from Thermococcus litomlis) are commonly used in polymerisation of nucleotides during polymerase chain reaction. In case of Taq polymerase the optimum temperature for polymerisation is 70-75°C because the base pairing between the primers and template DNA is much more specific at this temperature than that of 37°C. (The optimum temperature for E. coli DNA polymerase). - Trichoderma is used commercially to produce the enzyme cellulase, which is used to remove cell wall of plant cells. - Streptococcus pyrogenes responsible for many skin, and soft tissue infections.


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