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DUMET Medical DUMET Medical Solved Paper-2010

  • question_answer
     A mixture containing DNA fragments, a,b,c and d, with molecular weights of a + b = c, a > b and d > c, was subjected to agarose gel electrophoresis. The positions of these fragments from cathode to anode sides of the gel would be.

    A)  b,a,c,d

    B)  a,b,c,d

    C)  c,b,a,d

    D)  b,a,d,c

    Correct Answer: C

    Solution :

    Non-restrictive agarose gels are used for the separation of high molecular weight proteins and of a  large fragments of DNA. In the given case, when DNA fragments a b, c and are subjected to agarose gel electrophoresis, the position of given fragments from cathode to anode sides will be c, b, a, d (according to  their molecular weight).


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