A) its shape and structure
B) pH of medium
C) temperature
D) All of the above
Correct Answer: D
Solution :
Functionality of protein depends on its shape and structure, pH of medium, temperature. Note At low pH, Zwitter structure changes into cation but at high pH, Zwitter ion structure changes into anion. At high temperature, protein becomes denaturated. \[\underset{\left( at\text{ }high\text{ }pH \right)}{\mathop{N{{H}_{2}}-\overset{\begin{smallmatrix} R \\ | \end{smallmatrix}}{\mathop{C}}\,H-CO{{O}^{-}}}}\,\underset{O{{H}^{-}}}{\overset{{{H}^{+}}}{\longleftrightarrow}}\underset{Zwitter\text{ }ion}{\mathop{\overset{\oplus }{\mathop{N}}\,{{H}_{3}}-\overset{\begin{smallmatrix} R \\ | \end{smallmatrix}}{\mathop{C}}\,H-CO{{O}^{\Theta }}}}\,\] \[\underset{O{{H}^{-}}}{\overset{{{H}^{+}}}{\longleftrightarrow}}\underset{(at\,\operatorname{low}\,pH)}{\mathop{\overset{+}{\mathop{N}}\,{{H}_{3}}-\overset{\begin{smallmatrix} R \\ | \end{smallmatrix}}{\mathop{C}}\,H-COOH}}\,\]
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