A) buoyant density centrifugation
B) density gradient centrifugation
C) chromatography
D) electrophoresis
Correct Answer: A
Solution :
The buoyant density is defined as, the density at which a macromolecule will reach an equilibrium with the suspending medium, this is used for the separation of macromolecules. This method is so sensitive that it allows even separation of molecules having heavy isotopes. Density gradient centrifugation is used to separate cell organelles from cell homogenate. Chromatography is employed for the separation of constituents of mixture, and based upon the principle of partition coefficient. It is particularly used for the sciarion and purification of materials ranging from plant pigments to ammo acids. Electrophoresis is a technique for separating molecules of a mixture under the influence of an applied electric field.
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