| A. Ability to multiply and express outside the host in a bioreactor |
| B. A highly active promoter |
| C. A site at which replication can be initiated |
| D. One or more identifiable marker genes |
| E. One or more unique restriction sites |
A) A, C, D and E only
B) A, C and E only
C) B, C, D and E only
D) B, C and E only
Correct Answer: C
Solution :
: Plasmids are extra chromosomal DNA present naturally in a bacterial cell. They are small double-stranded and closed circular DNA. They are used in recombinant DNA technology as cloning and expression vectors. For acting as a vehicle for the desired gene, they should be able to multiply inside the host cell thus need a replication origin site, should have marker genes and unique restriction sites.
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