AIIMS AIIMS Solved Paper-2006

  • question_answer
    The function of leghaemoglobin during biological nitrogen fixation in root nodules of legumes, is to:

    A)  convert atmospheric \[{{N}_{2}}\] to \[N{{H}_{3}}\]

    B)  convert ammonia to nitrite

    C)  transport oxygen for activity of nitrogenase

    D)  protect nitrogenase from oxygen

    Correct Answer: D

    Solution :

     In the nodule, precise \[{{O}_{2}}\] levels are controlled by the \[{{O}_{2}}\] binding protein leghaemoglobin. This red iron containing protein is present in healthy nitrogen fixing nodules of leguminous plants. Leghaemoglobin functions as an ?oxygen buffer? cycling between the oxidized \[(F{{e}^{+3}})\] and reduced \[(F{{e}^{+2}})\] forms to keep free \[{{O}_{2}}\] levels within the ovule low. The ratio of leghaemoglobin bound \[{{O}_{2}}\] to free \[{{O}_{2}}\] in the root nodule is on the order of 10, 000 : 1. Nitrogenase enzyme, presents in root nodules mainly takes part in nitrogen fixation process. This enzyme becomes inactive in higher concentration of \[{{O}_{2}}\].


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