A) B - Denaturation at a temperature of about \[98{}^\circ C\] separating the two DNA strands.
B) A - Denaturation at a temperature of about \[50{}^\circ C.\]
C) C - Extension in the presence of heat stable DNA polymerase.
D) A - Annealing with two sets of primers.
Correct Answer: C
Solution :
[c] PCR is a technique for enzymatically replicating DNA without using a living organism such as E. coli or yeast. It is commonly used in medical and biological research labs for a variety of tasks like detection of hereditary diseases, identification of genetic fingerprints etc. The correct steps shown in the above figure are: A - Denaturation at a temperature of about \[94{}^\circ \] to \[98{}^\circ C.\] During the denaturation, the double strand melts open to single stranded DNA, and all enzymatic reactions stop. B - Annealing (binding of DNA primer to the separated strands. Occurs at \[50{}^\circ \] to\[65{}^\circ \]Celsius, which is lower than the optimal temperature of the DNA polymerases) C - Extension or elongation of the strands using the DNA primer with heat-stable DNA polymerases, most frequently Taq (Thermus aquaticus) at \[72{}^\circ C.\]
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