A) Denaturation of DNA on the gel for hybridization with specific probe.
B) Production of a group of genetically identical cells.
C) Digestion of DNA by restriction enzyme.
D) Denaturation of DNA from a nucleated cell such as the one from the scene of crime.
Correct Answer: C
Solution :
[c] The Southern blot is used to detect and identify certain DNA sequences in a sample of bodily fluid. It uses single-stranded DNA to search out their complementary strands. When a Southern blot is performed on DNA, the first step is digestion of DNA with restriction enzymes. Restriction enzymes cut DNA at known sequences, and produces DNA fragments of a certain length. Once the DNA is cut into pieces, scientists conduct electrophoresis to separate them by size.
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