| (i) For producing a recombinant protein (for therapeutic purpose) in large scale, which vector would you choose-a low copy number or hight copy number? |
| (ii) Would you like to choose an exonuclease enzyme while producing a recombinant DNA molecule? |
Answer:
(i) We choose a high-copy number, because higher the copy number, higher will be the formation of recombinant protein.
(ii) No, as exonuclease acts on the free ends of linear DNA molecule. Therefore, instead of producing DNA fragments with sticky ends, it will shorten or completely degrade the DNA fragment containing the gene of interest, and the circular plasmid (vector) will not get cut as it lacks free ends.
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