question_answer

Following are the steps of southern blot procedure.

(i) Autoradiography

(ii) Hybridisation with radioactive nucleic acid (probe).

(iii) Blotting

(iv) DNA fragments are treated to make them single stranded.

(v) Electrophoresis

(vi) Clearing of DNA by restriction endonuclease.

(vii) Isolation of DNA from sample

The correct sequence is:

A) (i), (ii), (iii), (iv), (v), (vi), (vii)

B) (vii), (vi), (v), (iv), (iii), (ii), (i)

C) (i), (ii), (vi), (vii), (iii), (iv), (v)

D) (vii), (vi), (i), (ii), (v), (iii), (iv)