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question_answer1)
Restriction endonucleases:
A)
are enzymes that process pre-RNA. done
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B)
are enzymes that degrade DNA. done
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C)
protect bacterial cells from viral infection. done
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D)
None of the above. done
clear
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question_answer2)
A gene is said to be cloned if:
A)
the DNA sequence of the gene is known. done
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B)
the function of the gene is known. done
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C)
there is a DNA probe for the gene. done
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D)
the gene has been isolated and copied. done
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question_answer3)
Which of the following is a critically important tool used in experiments involving DNA hybridisation?
A)
DNA sequencing machines done
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B)
Ligases done
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C)
DNA probes done
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D)
Vectors done
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question_answer4)
Introduction of one or more genes into an organism which normally does not possess them or their deletion by using artificial mean (not by breeding) comes under:
A)
Molecular biology done
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B)
Cytogenetics done
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C)
Genetic hybridization done
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D)
Genetic engineering done
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question_answer5)
A genetic marker is a:
A)
place where a restriction enzyme cuts DNA. done
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B)
chart that traces the family history of a genetic trait. done
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C)
nucleotide sequence near a particular gene. done
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D)
radioactive probe used to find a gene. done
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question_answer6)
A nucleic acid probe might be used to:
A)
insert genes into a host cell. done
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B)
make DNA for gene cloning. done
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C)
splice pieces of DNA. done
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D)
identify complementary segments present in nucleic acid sequence. done
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question_answer7)
DNA fragments with sticky ends are not allowed to undergo self ligation by:
A)
Unwindase done
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B)
Single strand binding proteins done
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C)
Gyrase done
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D)
Alkaline phosphatase done
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question_answer8)
In genetic engineering, a chimera is:
A)
an enzyme that links DNA molecules. done
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B)
a pasmid that contains foreign DNA. done
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C)
a virus that infects bacteria. done
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D)
a fungi. done
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question_answer9)
Restriction enzymes are used in genetic engineering because:
A)
they can join different DNA fragments. done
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B)
they can cleave DNA at a specific target. done
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C)
they are nucleases that cut DNA at variable sites. done
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D)
they are proteolytic enzymes which can degrade harmful enzymes. done
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question_answer10)
Which of the following is known as specific molecular scissors?
A)
Ligase done
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B)
Helicase done
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C)
Restriction endonuclease done
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D)
DNA polymerase done
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question_answer11)
The RTF region enables the plasmid to:
A)
be transmitted to otherbacteria by conjugation. done
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B)
undergo transformation. done
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C)
replicate in the host cell. done
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D)
code for enzymes that inactivate specific drugs. done
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question_answer12)
Which one of the following option is correct for 1, 2, 3 and 4?
A)
1-Exonuclease; 2-Endonuclease; 3-DNA ligase; 4-Transformation done
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B)
1-Endonuclease; 2-Exonuclease; 3-DNA ligase; 4-Transformation done
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C)
1-Exonuclease; 2-Endonuclease; 3- Hydrolase; 4-Transduction done
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D)
1-Restriction endonuclease; 2-Restriction endonuclease; 3-DNA ligase; 4-Transformation done
clear
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question_answer13)
Match column-I with column-II and choose the correct option.
| Column-I | | Column-II |
A. | Eco RI | I. | Bacillus amyloliquefaciens |
B. | Bam HI | II. | Haemophilus influenza |
C. | Hind III | III. | Escherichia coli |
D. | pBR 322 | IV. | Artificial plasmid |
A)
A-III; B-I; C-II; D-IV done
clear
B)
A-II; B-I; C-IV; D-III done
clear
C)
A-IV; B-I; C-III; D-II done
clear
D)
A-I; B-IV; C-II; D-III done
clear
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question_answer14)
Temperature required for various steps of polymerase chain reaction (i), (ii) and (iii) are:
(i) Denaturation (ii) Annealing |
(iii) Extension |
A)
(i) \[-94{}^\circ C\], (ii) \[-60{}^\circ C\], (iii) \[-72{}^\circ C\] done
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B)
(i)\[-40{}^\circ C\], (ii) \[-72{}^\circ C\], (iii) \[-94{}^\circ C\] done
clear
C)
(i) \[-72{}^\circ C\] (ii) - 94°C, (iii) \[-40{}^\circ C\] done
clear
D)
(i) \[-90{}^\circ C\], (ii) \[-72{}^\circ C\], (iii) \[-40{}^\circ C\] done
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question_answer15)
A \[{{T}_{4}}\] bacteriophage has a gene for the enzyme, lysozyme. The function of this enzyme is to digest:
A)
Cell wall done
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B)
Cell membrane done
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C)
Golgi done
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D)
Plasmid done
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question_answer16)
Most sensitive technique to detect malignant cell in non-Hodgkin's lymphoma is:
A)
Polymerase chain reaction done
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B)
Gene therapy done
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C)
Stem cell therapy done
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D)
None of the above done
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question_answer17)
In microinjection method, the DNA is incorporated into host cell by using:
A)
Micro-pipettes done
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B)
Micro-needles done
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C)
High voltage electric impulse done
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D)
Both [a] and [b] done
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question_answer18)
Which of the following statement is incorrect?
A)
Mobile genetic elements, tranposons were visualised by Barbara McClintock. done
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B)
Udder cell and somatic cell is used to produce the cloned sheep by nuclear transplantation method. done
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C)
In pedigree analysis, a person immediately affected by and action is called propositus. done
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D)
DNA ligases are used to cleave a DNA molecule. done
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question_answer19)
Which one of the following pairs of terms/names means one and the same thing?
A)
Gene pool of an organism-genome done
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B)
Codon-gene done
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C)
Cistron-triplet done
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D)
DNA fingerprinting-DNA profiling done
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question_answer20)
Match column-I with column-II and select the correct answer using the codes given below.
| Column-I | | Column-II |
A. | Southern blotting | I. | Running of DNA fragments on get. |
B. | Gel electrophoresis | II. | Cutting of DNA into fragments. |
C. | Cleavage | III. | Transfer of DNA |
D. | DNA probing | IV. | Searching for desired DNA fragments |
A)
A-IV; B-I; C-II; D-III done
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B)
A-III; B-I; C-II; D-IV done
clear
C)
A-IV; B-I; C-III; D-II done
clear
D)
A-I; B-IV; C-II; D-III done
clear
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question_answer21)
Polyethylene glycol method is used for:
A)
biodiesel production. done
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B)
seedless fruit production. done
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C)
energy production from sewage. done
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D)
gene transfer without a vector. done
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question_answer22)
Plasmids are suitable vectors for gene cloning because:
A)
these are small circular DNA molecule which can integrate with host chromosomal DNA. done
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B)
these are small circular DNA molecules with their own replication origin site. done
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C)
these can shuttle between prokaryotic and eukaryotic cells. done
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D)
these often carry antibiotic resistance genes. done
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question_answer23)
Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using:
A)
BACs done
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B)
Expression vectors done
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C)
YACs done
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D)
All of these done
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question_answer24)
Which of the following is not a genetic vector?
A)
Plasmid done
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B)
Phage done
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C)
Cosmid done
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D)
Virusoid done
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question_answer25)
The introduction of TDA into plants involves:
A)
exposing the plants to cold for a brief period. done
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B)
allowing the plant roots to stand in water. done
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C)
infection of the plant by Agrobacterium tumefaciens. done
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D)
None of these done
clear
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question_answer26)
Due to chloramphenicol resistance gene, one is able to select a transformed cell in the presence of chloramphenicol. The chloramphenicol resistance gene in this case is called:
A)
Origin of replication done
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B)
Cloning sites done
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C)
Selectable marker done
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D)
None of the above done
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question_answer27)
Electrophoresis is used to:
A)
separate fragments of DNA. done
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B)
clone genes. done
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C)
cut DNA into fragments. done
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D)
match a gene with its function. done
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question_answer28)
A biologist intends to use a polymerase chain reaction to perform a genetic task. The biologist probably is trying to:
A)
discover new genes. done
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B)
clone a gene. done
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C)
cut DNA into many small fragments. done
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D)
isolate DNA from a living cell. done
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question_answer29)
Consider the given figure and identify the correct option.
A)
A - Denaturation, B - Annealing, C - Extension, D ? Amplified done
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B)
A - Annealing, B - Deoaturation, C - Extension, D ? Amplified done
clear
C)
A- Denaturation, B -Annealing, C -Amplified, D ? Extension done
clear
D)
A-Annealing, B - Denaturation, C -Amplified, D ? Extension done
clear
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question_answer30)
Which one of the following pairs is not correctly matched?
A)
Plasmid - Small piece of extra chromosomal DNA in bacteria done
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B)
Interferon - An enzyme that interferes with DNA replication done
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C)
Cosmid - A vector for carrying large DNA fragments into host cells done
clear
D)
Myeloma - Antibody - producing tumour cells done
clear
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question_answer31)
Which of the following is/are related to biotechnology?
(i) In vitro fertilisation. |
(ii) Synthesis of a gene. |
(iii) Correcting a defective gene. |
(iv) Developing a DNA vaccine. |
A)
(i) and (ii) done
clear
B)
(ii) and (iii) done
clear
C)
(iii) and (iv) done
clear
D)
(i), (ii), (iii) and (iv) done
clear
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question_answer32)
Match column-I with column-II and choose the correct option.
| Column-I | | Column-II |
A. | Recombinant DNA | I. | Sea Weeds |
B. | Ethidium bromide | II. | DNA staining |
C. | Gel electrophoresis | III. | Plasmid DNA that has incorporated into human |
D. | Agarose | IV. | Process by which DNA fragments are separated based on their size |
A)
A-III; B-II; C-IV; D-I done
clear
B)
A-III; B-II; C-I; D-IV done
clear
C)
A-II; B-I; C-IV; D-III done
clear
D)
A-III; B-IV; C-I; D-II done
clear
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question_answer33)
DNA fragments are separated using gel electrophoresis:
A)
because DNA is pulled through the gel toward the negative end of the field. done
clear
B)
because larger DNA fragments move faster through the gel than smaller DNA fragments. done
clear
C)
to identify and isolate DNA fragments. done
clear
D)
to synthesise DNA for cloning. done
clear
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question_answer34)
Complementary base pairing is important for:
A)
ligation reactions with blunt-end DNA molecules. done
clear
B)
hybridization between DNA and transcription factors. done
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C)
restriction endonucleases to cut cell walls. done
clear
D)
synthesising cDNA molecules from mRNA templates. done
clear
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question_answer35)
In genetic engineering, genes can be inserted from one organism into another or back into the original organism by using which of the following techniques?
A)
Polymerase chain reaction done
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B)
Gene gun done
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C)
DNA hybridization done
clear
D)
Gel electrophoresis done
clear
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question_answer36)
A restriction fragment containing a specific gene of interest can be identified by gel electrophoresis followed by transferring the DNA to a membrane as solid support matrix using a procedure called:
A)
Gene amplification done
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B)
Southern blotting done
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C)
Identification of a gene done
clear
D)
Me Clintock done
clear
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question_answer37)
Restriction endonuclease - Hind. II always cuts DNA molecules at a particular point by recognising a specific sequence of:
A)
six base pairs done
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B)
five base pairs done
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C)
four base pairs done
clear
D)
seven base pairs done
clear
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question_answer38)
Which of the following is/are used in recombinant DNA technology?
(i) Agarose gel |
(ii) Ethidium bromide |
(iii) Plasmid vector |
(iv) Restriction endonuclease |
A)
(i) and (ii) done
clear
B)
(ii) and (iii) done
clear
C)
(iii) and (iv) done
clear
D)
(i), (ii), (iii) and (iv) done
clear
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question_answer39)
Which of the following statements about restriction enzymes is incorrect?
A)
They work on DNA extracted from all types of organisms. done
clear
B)
They are used to glue short segments of DNA. done
clear
C)
They come in many varieties, each with its own DNA target sequence. done
clear
D)
They are highly specific for their DNA target sequences. done
clear
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question_answer40)
Which of the following statements represent/s 'cloning'?
A)
all of the cells are derived from one cell and are genetically identical. done
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B)
a gene from one organism has been inserted into a vector and successfully introduced into a host cell. done
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C)
all of the cells in a particular organism are identical. done
clear
D)
All of the above done
clear
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question_answer41)
Recombinant DNA can be transferred into host cell by:
A)
growing the host cell in growth medium containing ampicillin. done
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B)
coating the DNA with carbohydrates so that the cells will engulf the DNA. done
clear
C)
treating cells with calcium ions or electrical pulses to increase cell permeability. done
clear
D)
injecting proteins into host cells to make them more permeable. done
clear
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question_answer42)
Identify the correct statements.
(i) The first recombinant DNA was constructed by using a piece of DNA from a plasmid carrying antibiotic resistance gene in the bacterium Salmonella typhimurium and linked it to the plasmid of E. coli. |
(ii) Cohen and Boyer are known as father of genetic engineering. |
(iii) When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends and these can be joined together using DNA ligases. |
(iv) Endonucleases remove nucleotides from the ends of the DNA whereas exonucleases make cuts at specific positions within the |
DNA. |
(v) Presence of more than one recognition sites within the vector will generate several fragments which will complicate the gene cloning. |
(vi) Humulin was the first recombinant DNA based product, produced and marketed in India. |
(vii) YAC vectors contain the telomeric sequence, the centromere and autonomously replicating sequence from yeast chromosomes. |
(viii)Alkaline phosphatase, is used to prevent unwanted self ligation of the vector DNA molecules in procedures of rDNA technology. |
(ix) pBR 322 vector was the first artificial ideal vector constructed by Boliver and Rodriguez. |
(x) Plasmid DNA is coated with histone proteins and can act as genetic factor. |
A)
(ii), (iv), (vi) and (x) done
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B)
(ii), (iv), (v) and (x) done
clear
C)
(i), (iii), (v), (vii), (viii) and (ix) done
clear
D)
(i), (ii), (iv), (vi) and (x) done
clear
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