A) Recombinant DNA technique with the help of E. coli
B) Two coded insulin genes separated then incorporated into bacteria
C) The extraction of cow's and pig's pancreas
D) Technique not developed till now
Correct Answer: A
Solution :
Eli Lily (American company) in 1893 produced genetically engineered insulin called humulin with the help of E. coli, plasmid clone.
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