Answer:
RNAi technology is used to block the
expression of certain genes and also referred to asgene silencing. During this
process, a complementary RNA to the mRNA being produced by the gene is
introduced into the cell. This RNA binds to the mRNA making it double stranded
and therefore, stops the process of translation. e.g., a nematode
Meloidegyneincognitia infects the roots of tobacco plants which reduces the
production of tobacco.
It can be prevented by using RNA
interference (RNAi) process which is checked by silencing of specific mRNA due
to a complementary dsRNA.
dsRNA binds and prevents the
translation of mRNA (silencing). By using Agrobactenum vectors,
Nematode-specific genes were introduced into the host plants which produces both
sense and anti-sense RNA in the host cells.
These two RNAs are complementary
to each other and form a double-stranded RNA
(dsRNA) that initiates RNAi and
hence, silence the specific mRNA of the nematode. The parasite cannot survive
in transgenic host and so prevents the plants from pests.
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