12th Class Biology Biotechnology : Principles And Processes

  • question_answer 44)
      A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing there combinant DNA. How does this affect the next step in the experiment i.e., bacterial transformation?


                      The experiment will not likely to be affected as recombinant DNA molecule is circular and closed, with no free ends. Hence, it will not be a substrate for exo nuclease enzyme which removes nucleotides from the free ends of DNA.

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