12th Class Biology Principals And Processes Of Biotechnology

  • question_answer 44)   A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing there combinant DNA. How does this affect the next step in the experiment i.e., bacterial transformation?

    Answer:

                      The experiment will not likely to be affected as recombinant DNA molecule is circular and closed, with no free ends. Hence, it will not be a substrate for exo nuclease enzyme which removes nucleotides from the free ends of DNA.

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