Manipal Medical Manipal Medical Solved Paper-2010

  • question_answer
    The enzyme employed for amplification of DNA during PCR is commercially obtained from

    A)  Streptococcus pyrogenes

    B)  Bacillus licheniformis

    C)  Trichoderma reesi

    D)  Thermus aquaticus

    Correct Answer: D

    Solution :

     Heat stable DNA polymerase, ie, Taq (isolated from Thermus acquaticus), Pfu (from Pyrococcus funosus) and vent (from Thermococcus Utoralis) are commonly used in polymerisation of nuclsotides during polymerase chain in reaction. In case of Taq polymerase, the optimum temperature for polymerisation is\[70-75{}^\circ C,\] because the base pairing between the primers and template DNA is much more specific at this temperature than that of\[37{}^\circ C\] (The optimum temperatre for E. coli DNA polymerase.) Trichoderma is used commercially to produce the enzyme cellulase, which is used to remove cell wall of plant cells. Streptococcus pyrogenes is responsible for many skin, and soft tissue infections.


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