question_answer

Why cloning sites are required in a - vector? What will happen if many recognition sites are present within the vector?

Answer:

Cloning sites are required to link foreign DNA with the vector. These sites are also called recognition sites as these are recognised by restriction enzymes. An ideal vector should have very few or single cloning site. If more than one recognition sites are present within the vector, it will generate several fragments. It will lead to more complication in gene cloning.