question_answer

(i) State the type of technique shown in the diagram and its importance.

(ii) Identify the step occurring in the middle and its utility.

(iii) Mention the role of tag polymerase in the technique.

Answer:

(i) The technique shown in the given diagram is Polymerase Chain Reaction (PCR), which is used in Recombinant DNA Technology (RDT) and various other fields to amplify a small segment of DNA.

(ii) The second step of PCR is annealing (primer extension), in which two small oligonucleotide primers are used to anneal or hybridize with the single - stranded DNA template.

(iii) Taq polymerase is a thermos table enzyme, which is used in the polymerase chain reaction because it can tolerate the high temperature which is required to carry out the synthesis of new DNA.