question_answer

                                  Diagrammatically represent the schematic sketch of replication fork of DNA. Discuss the importance of different enzymes involved in its replication.

Answer:

The whole DNA does not open in one stretch due to very high energy requirement, so the replication occurs within a small opening of the DNA helix, which gives the appearance of a Y-shaped structure known as replication fork.

DNA replication requires a set of different enzymes which are as follows

(i) DNA helicase It helps to unwind the DNA strand for the formation of a replication fork.

(ii) DNA dependent DNA polymerase This enzyme uses a DNA template (parental strands) to catalyse the polymerisation of deoxyribonucleotides.

(iii) DNA ligase It facilitates the joining of discontinuously synthesised DNA fragments known as Okazaki fragments.