question_answer

                                                              PCR is used for the amplification of DNA. Explain. Also, give the applications of PCR in any two areas.

Answer:

PCR is the technique of amplifying the gene of interest. It is basically in vitro DNA replication. It is based on the principle that a DNA molecule, when subjected to high temperature splits into its two strands due to denaturation. Each DNA strand now serves as a template for synthesis of new DNA strands. Thus, the extension product of one cycle serves as a template for subsequent cycles. As a result, each cycle essentially doubles the amount of DNA from previous cycle.      

Applications of PCR Some areas of its applications are as follows

· Detection of pathogens in clinical specimens obtained from infected body part.

· DNA fingerprinting.