question_answer

A vector is engineered with three features which facilitate its cloning within the host cell. List these three features.

Answer:

The features required to facilitate cloning of a vector into host cell are

(a) Origin of replication (ori) It is a sequence from where the replication of DNA initiates. It also controls the copy number of the DNA linked to it.

(b) Selectable marker It helps in identification of trans formants and elimination of non-trans formant cells by selectively permitting growth of transformed cells. e.g \[Te{{t}^{r}},\text{ }Am{{p}^{s}}\]

(c) Cloning sites These link the foreign DNA to the vector DNA. These are identified by restriction enzymes and specifically cut to generate fragments.