question_answer

The recovery and identification of genes and other specific DNA sequences by cloning or by PCR is a powerful tool for analyzing genomic structure and function. One of the first steps in characterizing a DNA clone is the construction of a restriction map. A restriction map is a compilation of the number, order and distance between restriction enzyme-cutting sites along a cloned segment of DNA. Study the given figure showing the restriction fragments separated by gel electrophoresis and construct the restriction map.

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