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question_answer1)
Maximum utilisation of the techniques of biotechnology has been made in the field of:
A)
Agriculture done
clear
B)
Medicines done
clear
C)
Industries done
clear
D)
Biogas production done
clear
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question_answer2)
Golden rice is a transgenic crop of the future with the following improved trait:
A)
High lysine (essential amino acid) content done
clear
B)
Insect resistance done
clear
C)
High protein content done
clear
D)
High vitamin-A content done
clear
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question_answer3)
The bacteria generally used for generic engineering is:
A)
Agrobacterium done
clear
B)
Bacillus done
clear
C)
Pseudomonas done
clear
D)
Clostridium done
clear
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question_answer4)
Polyploidy can result from:
A)
Double fertilisation done
clear
B)
Polyspermy done
clear
C)
Diploid gametes done
clear
D)
All of these done
clear
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question_answer5)
Strains of Bacillus thuringiensis are used in producing:
A)
Bioinsecticidal plants done
clear
B)
Biomineralisation done
clear
C)
Biometallurgical techniques done
clear
D)
Biofertilisers done
clear
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question_answer6)
Which is not a transgenic plant?
A)
Soyabean done
clear
B)
Maize done
clear
C)
Golden rice done
clear
D)
Cucumber done
clear
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question_answer7)
The pesticide most persistent in the soil is:
A)
DDT done
clear
B)
BHC done
clear
C)
Dieldrin done
clear
D)
Baygon done
clear
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question_answer8)
The crops having cry genes need:
A)
no insecticide done
clear
B)
mild quantity of insecticide done
clear
C)
large amount of insecticide done
clear
D)
5 kg insecticide / hectare done
clear
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question_answer9)
cry n Ab and cry I Ab produce toxins that control:
A)
cotton bollworms and corn borer respectively done
clear
B)
cotton borer and cotton bollworms respectively. done
clear
C)
tobacco budworms and nematodes respectively. done
clear
D)
nematodes and tobacco budworms respectively. done
clear
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question_answer10)
Which of the following Bt crops is being grown in India by the farmers?
A)
Cotton done
clear
B)
Brinjal done
clear
C)
Soyabean done
clear
D)
Maize done
clear
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question_answer11)
A GM crop is transgenic. It can be harmful because it may cause:
A)
allergies and toxicity. done
clear
B)
incorporation of antibiotic resistance in human beings. done
clear
C)
disturbance in metabolism due to enzyme for antibiotic resistance. done
clear
D)
All of the above done
clear
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question_answer12)
Flavr savr variety of Tomato is improved variety developed through incorporation of a transgene. Flavr savr variety of tomato remains fresh for long because it:
A)
has little polygalacturonase done
clear
B)
abundant polygalacturonase done
clear
C)
has gene for antibiotic resistance done
clear
D)
lacks amylase done
clear
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question_answer13)
Which of the following is/are correct?
(i) | Biowar | Biowar is the use of biological weapons against humans and or their crops and animals. |
(ii) | Bioethics | Bioethics is the unauthorized use of bioresources and traditional knowledge related to bioresources for commercial benefits. |
(iii) | Biopatent | Exploitation of bioresources of other nations without proper authorisation. |
A)
Only (ii) done
clear
B)
Only (i) done
clear
C)
(i) and (ii) done
clear
D)
(i) and (iii) done
clear
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question_answer14)
Match column-I with column-II and choose the correct option.
| Column-I | | Column-II |
A. | Escherichia coli | I. | nif gene |
B. | Bacillus | II. | Interferon |
C. | Rhizobium meliloti | III. | Bt toxin |
D. | Pseudomonas putida | IV. | Bioremediation |
A)
A-II; B-I; C-IV; D-III done
clear
B)
A-II; B-I; C-III; D-IV done
clear
C)
A-II; B-III; C-I; D-IV done
clear
D)
A-IV; B-I; C-II; D-III done
clear
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question_answer15)
Which one of the following statement is correct?
A)
The proteins encoded by the genes cry I Ac and cry II Ab control cotton bollworms. done
clear
B)
Protein encoded by cry I Ab controls corn borer. done
clear
C)
Both [a] and [b] done
clear
D)
Proteins encoded by cry I Ac and cry I Ab control flies. done
clear
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question_answer16)
Modifications by germ line gene therapy are heritable as:
A)
the functional gene is incorporated into their genome. done
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B)
the functional gene is incorporated into one of the gene. done
clear
C)
the functional gene is incorporated into somatic cells. done
clear
D)
All of the above done
clear
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question_answer17)
Biotechnology deals with industrial scale production of biopharmaceuticals and biological products using genetically modified:
A)
microbes only done
clear
B)
plants and animals only done
clear
C)
fungi only done
clear
D)
All of the above done
clear
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question_answer18)
Demerit of bovine and porcine insulin is:
A)
Hypercalcemia done
clear
B)
Expensive done
clear
C)
Allergic reactions done
clear
D)
Mutations in human genome done
clear
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question_answer19)
................. and, ............... are present at N and C terminal of \[\beta \] chain of threonine.
A)
Phenylalanine and alanine done
clear
B)
Glycine and asparagine done
clear
C)
Leucine and isoleucine done
clear
D)
Methionine and tryptophan done
clear
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question_answer20)
In the treatment of SCID, cDNA is introduced into a patient's cells using.............as a vector.
A)
E coli done
clear
B)
Retrovirus done
clear
C)
Bacillus thuringiensis done
clear
D)
Agrobacterium done
clear
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question_answer21)
Select the correct set of the names for 1, 2, 3 and 4.
A)
B)
C)
D)
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question_answer22)
Match column-I with column-II and choose the correct option.
| Column-I | | Column-II |
A. | Golden rice | I. | Increased shelf life |
B. | Flavr Savr | II. | hGH |
C. | Mouse | III. | Vitamin A |
D. | Transgenic pig | IV. | Organ transplantation |
A)
A-III; B-I; C-II; D-IV done
clear
B)
A-II; B-I; C-III; D-IV done
clear
C)
A-II; B-III; C-I; D-IV done
clear
D)
A-IV; B-I; C-II; D-III done
clear
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question_answer23)
Which of the following statement is incorrect?
(i) Recombinant DNA technology is used to improve crop plants by increasing their productivity, by making them more nutritious and by developing disease resistant. |
(ii) Bt cotton is resistant to bollworm infestation. |
(iii) Bacillus thuringiensis form cry protein during any phase of their growth. |
(iv) Bacillus thuringiensis is not harmed by self cry protein because of its occurrence as protoxin (inactive). |
(v) Protoxin cry protein is changed into active cry protein in the stomach of insects due to alkaline pH in stomach. |
A)
(i), (ii) and (iv) done
clear
B)
(i) and (iv) done
clear
C)
Only (iii) done
clear
D)
All of the above done
clear
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question_answer24)
Multicellular organisms that carry a specific genetic change in each cell because of an intervention at the embryo level is a:
A)
Transversion done
clear
B)
Transition done
clear
C)
Transgenic done
clear
D)
Transformant done
clear
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question_answer25)
One of the following are transgenic organisms.
A)
Holly sheep and Tomato done
clear
B)
Dolly sheep and Subabul done
clear
C)
Molly sheep and Banana done
clear
D)
Bt Cotton and Tomato (Flavr Savr) done
clear
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question_answer26)
Bioaugmentation is:
A)
the addition of commercially prepared catabolic activated bacterial strains to degrade wastes. done
clear
B)
the production of fertilisers by using bacteria and virus done
clear
C)
the metals are deposited as insoluble oxides and sulphides by activity of bacteria. done
clear
D)
the removal of pests. done
clear
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question_answer27)
Which was the first product contributed to human kind by science of biotechnology in early 1970's?
A)
Somatotropin done
clear
B)
Insulin done
clear
C)
Cytokines done
clear
D)
Erythropoietin done
clear
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question_answer28)
Most widely used bioweapon is:
A)
Bacillus subtilis done
clear
B)
Pseudomonas putida done
clear
C)
Bacillus anthracis done
clear
D)
None of the above done
clear
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question_answer29)
In a criminal trial in which DNA fingerprinting has been used, a genetic "match" between the suspect and evidence left at the crime scene provides:
A)
definitive proof that the suspect is guilty. done
clear
B)
a high probability that the suspect is guilty. done
clear
C)
a low probability that the suspect is guilty. done
clear
D)
definitive proof that the suspect is innocent. done
clear
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question_answer30)
Yersinia pestis is used as biological weapon. It causes a disease:
A)
Smallpox done
clear
B)
Plague done
clear
C)
Tularemia done
clear
D)
Anthrax done
clear
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question_answer31)
In 1921, two Canadian scientists Frederick G. Banting and Charles. H. Best successfully purified insulin from a:
A)
Pig's pancreas done
clear
B)
Camel's pancreas done
clear
C)
Dog's pancreas done
clear
D)
Cattle's pancreas done
clear
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question_answer32)
Which of the following biotechnological product can enhance the immune system?
A)
IL-2 done
clear
B)
GM-CSF done
clear
C)
TNF done
clear
D)
All of these done
clear
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question_answer33)
Protein engineering and drug designing requires CAMM (computer aided molecular modelling) because of:
A)
study of three dimensional structures of receptors and functional molecules. done
clear
B)
requirement of DNA recombinant technology. done
clear
C)
specificity of proteins. done
clear
D)
All of the above done
clear
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question_answer34)
Which of the following statements is incorrect?
(i) Insulin was originally extracted from pancreas of slaughtered pigs and cattle. |
(ii) Animal insulin is difficult to obtain. |
(iii) Animal insulin is identical to human insulin. |
(iv) Non human insulin caused some patients to develop allergy. |
(v) Recombinant DNA allowed scientists to insert a human insulin gene into a bacterial expression vector. |
(vi) Recombinant insulin is actually obtained from E. coli bacterial cell. |
A)
(i) and (ii) done
clear
B)
(iii) and (iv). done
clear
C)
Only (iii) done
clear
D)
Only (vi) done
clear
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question_answer35)
Match column-I with column-II and choose the correct option.
| Column-I | | Column-II |
A. | Gene therapy | I. | Rhizobium |
B. | Biofertiliser | II. | Cry gene |
C. | Bt cotton | III. | SCID |
D. | Humulin | IV. | Diabetes |
A)
A-II; B-I; C-IV; D-III done
clear
B)
A-III; B-I; C-II; D-IV done
clear
C)
A-II; B-III; C-I; D-IV done
clear
D)
A-IV; B-I; C-II; D-III done
clear
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question_answer36)
Match column-I with column-II and choose the correct option.
| Column-I | | Column-II |
A. | Bacillus Thuringiensis | I. | Production of chitinases |
B. | Rhizobium meliloti | II. | Scavenging of Oil Spill |
C. | Pseudomonas putida | III. | Incorporation of nif gene |
D. | Trichoderma | IV. | Production of Bt toxin |
| | V. | Production of human insulin |
A)
A-II; B-IV; C-I; D-V; E-III done
clear
B)
A-II; B-IV; C-V; D-I; E-III done
clear
C)
A-IV; B-III; C-V; D-II; E-I done
clear
D)
A-V; B-IV; C-III; D-I; E-II done
clear
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question_answer37)
Which of the following GM crop is produced by antisense RNA technology where the expression of native gene is blocked?
A)
Bt cotton done
clear
B)
Golden rice done
clear
C)
Flavr Savr done
clear
D)
Golden Mustard done
clear
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question_answer38)
The main use of recombinant DNA technology are:
A)
production of transgenic humans. done
clear
B)
the creation of cells capable of synthesising economically important molecules. done
clear
C)
the efficient reduction of useful proteins. done
clear
D)
Both [b] and [c] done
clear
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question_answer39)
.............infects the roots of tobacco plants which reduces the production of tobacco?
A)
Nematode (Meloidogyne incognita) done
clear
B)
Coleopterans (beetles) done
clear
C)
Lepidopterans (army worm) done
clear
D)
Dipterans (mosquitoes) done
clear
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question_answer40)
Golden rice was created by transforming rice with two beta-carotene biosynthesis genes, namely:
A)
Psy and cry I genes done
clear
B)
LCY-E done
clear
C)
CHY-1 done
clear
D)
CHY-2 done
clear
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question_answer41)
Humulin is:
A)
A form of chitin done
clear
B)
A new digestive enzyme done
clear
C)
A powerful antibiotic done
clear
D)
Human insulin done
clear
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question_answer42)
A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of:
A)
Pseudomonas done
clear
B)
Trichoderma done
clear
C)
Xanthomonas done
clear
D)
Bacillus done
clear
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question_answer43)
When a patient with defective ADA was treated, which of the following steps was performed for gene therapy?
(i) Lymphocytes were obtained from the patients. |
(ii) Lymphocytes are transferred to culture dishes. |
(iii) Lymphocytes were transfected with normal ADA genes. |
(iv) The transfected cells are returned to the patients. |
A)
All of the above done
clear
B)
(iii) and (iv) done
clear
C)
Only (iv) done
clear
D)
SCID cannot be treated done
clear
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question_answer44)
Following are the steps of southern blot procedure.
(i) Autoradiography |
(ii) Hybridisation with radioactive nucleic acid (probe). |
(iii) Blotting |
(iv) DNA fragments are treated to make them single stranded. |
(v) Electrophoresis |
(vi) Clearing of DNA by restriction endonuclease. |
(vii) Isolation of DNA from sample |
The correct sequence is: |
A)
(i), (ii), (iii), (iv), (v), (vi), (vii) done
clear
B)
(vii), (vi), (v), (iv), (iii), (ii), (i) done
clear
C)
(i), (ii), (vi), (vii), (iii), (iv), (v) done
clear
D)
(vii), (vi), (i), (ii), (v), (iii), (iv) done
clear
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question_answer45)
Match column-I with column-II and choose the correct option.
| Column-I | | Column-II |
A. | Virus free plant | I. | Rhizobium |
B. | Biofertiliser | II. | Cry gene |
C. | Bt cotton | III. | Shoot apex |
D. | Humulin | IV. | Diabetes |
A)
A-II; B-I; C-IV; D-III done
clear
B)
A-III; B-I; C-II; D-IV done
clear
C)
A-II; B-III; C-I; D-IV done
clear
D)
A-IV; B-I; C-II; D-III done
clear
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question_answer46)
DNA vaccines are:
A)
mixture of hormones done
clear
B)
recombinant vaccines done
clear
C)
synthetic vaccines done
clear
D)
pure DNA or RNA done
clear
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question_answer47)
The name of the drug used in cancer treatment produced by biotechnology is:
A)
Interferon done
clear
B)
Hgh done
clear
C)
TSH done
clear
D)
Insulin done
clear
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question_answer48)
By the use of biotechnology in which of the following bacteria production of \[{{B}_{2}}\]vitamins has been increased to about 20,000 times?
A)
Ashbya gossypii done
clear
B)
E. coli done
clear
C)
Pseudomonas denitrificans done
clear
D)
Propionibacterium shermanii done
clear
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question_answer49)
Recombinant DNA Technology prepared vaccine is called:
A)
First generation vaccine done
clear
B)
Second generation vaccine done
clear
C)
Third generation vaccine done
clear
D)
Synthesised vaccine done
clear
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question_answer50)
Human insulin is being commercially produced from a transgenic species of:
A)
Rhizobium done
clear
B)
Saccharomyces done
clear
C)
Escherichia done
clear
D)
Mycobacterium done
clear
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question_answer51)
Somatostatin or human growth hormone (HGH) for the first time was genetically isolated by:
A)
Banting and Best done
clear
B)
Ross done
clear
C)
Pasteur done
clear
D)
Goeddel done
clear
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question_answer52)
Hirudin gene inserted in Brassica napus was:
A)
synthesised chemically. done
clear
B)
obtained from leech. done
clear
C)
obtained from Bacillus thuringiensis. done
clear
D)
Brassica campestris. done
clear
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question_answer53)
Given below are certain characteristics of thurioside protein. Which of the statement given are correct?
(i) It is activated by alkaline pH of the gut of the insect pest ultimately killing it. |
(ii) It binds with the epithelial cells of the midgut of the insect pest ultimately killing it. |
(iii) It does not kill the carrier bacterium which is itself resistant to this toxin. |
A)
(i) and (ii) done
clear
B)
(i) and (iii) done
clear
C)
(ii) and (iii) done
clear
D)
(i), (ii) and (iii) done
clear
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question_answer54)
Following are the steps in one type of gene therapy.
(i) Inject engineered cells into patients bone marrow. |
(ii) Viral DNA earring the normal allele inserts into chromosome. |
(iii) Let retrovirus infect bone marrow cells that have removed from patient and cultured. |
(iv) Insert RNA version of normal allele into retrovirus. |
The correct sequence is: |
A)
(i), (ii), (iii) and (iv) done
clear
B)
(iv), (iii), (ii) and (i) done
clear
C)
(i), (ii), (iv) and (iii) done
clear
D)
(iv), (iii), (i) and (ii) done
clear
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question_answer55)
Select the correct statement.
A)
Genetic engineering works only on animals and has not yet been successfully used on plants. done
clear
B)
There are no risks associated with DNA technology. done
clear
C)
The first step in PCR is heating which is used to separate both the strands of target DNA. done
clear
D)
DNA from one organism will not bond to DNA from another animal. done
clear
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question_answer56)
Consider the following statements.
(i) Transgenic animals are more sensitive to toxic substance than non-transgenic animals. |
(ii) Useful biological products can be produced by introducing portion of DNA into transgenic animals which codes for a particular product. |
(iii) Brazzein is protein produced by a west African plant, Pentadiplandra brazzeana which is approximately 2000 times as sweet as sugar. |
Which of the statements are given above is correct? |
A)
(i), (ii) and (iii) done
clear
B)
(i) and (ii) done
clear
C)
(i) and (iii) done
clear
D)
None of these done
clear
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question_answer57)
Consider the following statements.
(i)In earlier days insulin extracted from pancreas of slaughtered cattle and pigs, was more efficient than genetically engineered insulin. |
(ii) PCR technique is being used for the detection of HIV and genetic mutations, in suspected cancer patients. |
(iii) Cystic fibrosis, heamophilia, cancer, Parkinson's etc., are treated by gene therapy. |
Which of the statements given above are correct? |
A)
(i) and (ii) done
clear
B)
(i) and (iii) done
clear
C)
(ii) and (iii) done
clear
D)
(i), (ii) and (iii) done
clear
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