question_answer 53)

  ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?

Answer:

                  Yes, one can use antibody against the enzyme (that is responsible for the metabolism of phenylanaline) to develop ELISA based diagnostic technique, the patient, in which the enzyme-protein complex is absent would give a negative result in ELISA when comparedto the normal individual.