In selection of recombinants due to
inactivation of antibiotics, the transformed cells are first plated on the antibiotic
plate which has not been insertionally inactivated (i.e., ampicillin)and
incubated overnight for growth of transformants.
For selection of recombinants, these
transformants are replica-plated on second antibiotic(say, tetracycline) plate
(which got inactivated due to insertion of gene),
Non-recombinants grow on both
the plates (one carrying ampicillin and the other carrying tetracycline) while
recombinants will grow only on ampicillin plate. This entire exercise is labourious
and takes more time (two overnight incubation) as well However, if we choose
insertional inactivation of a marker that produces colour in the presence of a
chromogenic compound, we can distinguish between the recombinants and non-recombinants
on a single medium plate (containing one antibiotic and the chromogenic,
compound) after overnight growth.
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