12th Class Biology Biotechnology : Principles And Processes

  • question_answer 52)
      Identify and explain A, B and C in the PCR diagram given below,  


                          Region to be amplified     In PCR, each cycle has three steps (i) Denaturation of DNA Sample Unwinding of two strand of DNA by heating the sample at92-94°C. (ii) Primer Annealing Primers get positioned on the exposed nucleotides as per base pairing rules. (iii) Extension of Primers DNA polymerase recognizes primers as 'start' tags and begins to extend the primers using the free nucleotides provided in the reaction and the genomic DNA as template. With each round of reactions, the DNA doubles.

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