Region to be amplified
In PCR, each cycle has three
(i) Denaturation of DNA
Sample Unwinding of two strand of DNA by heating the sample at92-94°C.
(ii) Primer Annealing
Primers get positioned on the exposed nucleotides as per base pairing rules.
(iii) Extension of Primers
DNA polymerase recognizes primers as 'start' tags and begins to extend the
primers using the free nucleotides provided in the reaction and the genomic DNA
With each round of reactions,
the DNA doubles.
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