The reasons are as follows DNA
sample that was loaded on the gel may have got contaminated with nudease(exo or
endo both) and completely degraded.
(ii) Electrodes were put in opposite
orientation in the gel assembly that is anode towards the wells (where DNA
sample is loaded). Since, DNA molecules are negatively charged, they move towards
anode and hence, move out of the gel instead of moving into the matrix of gel.
(iii) Ethidium bromide was not
added at all or was not added in sufficient concentration and so DNA was not
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