In a gene cloning experiment, first a
recombinant DNA molecule is constructed, where the gene of interest is ligated
to the vector (the step would not be affected) and introduced inside the host
Since, not all the cells get transformed
with the recombinant/plasmid DMA. In the absence of selectable marker, it will
be difficult to distinguish between transformants and non -transformant,
because role of selectable marker is in the selection of transformant.
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