12th Class Biology Principals And Processes Of Biotechnology

  • question_answer 46)   A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA b and, while linear DMA showstwo fragments. Explain.


                      When a plasmid DNA and a linear DNA having one site for a restriction endonuclease are cut and separated, plasmid shows one DNA band, while linear DNA shows two band because of difference in their basic structure. Plasmid is a circular DNA molecule and when cut with these enzymes, it becomes linear but does not get fragmented due to presence of only one restriction site, whereas a linear DNA molecule gets cut into two fragment.

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