12th Class Biology Principals And Processes Of Biotechnology

  • question_answer 22)   Which of the following has popularized the PCR (Polymerase Chain Reaction)? (a) Easy availability of DNA template                       (b) Availability of synthetic primers (c) Availability of cheap deoxyribonucleotides    (d) Availability of' Thermostable' DNA polymerase

    Answer:

                      (d) The Polymerase Chain Reaction (PCR) is a reaction in which amplification of specific DNA sequences is carried out in vitro. Such repeated amplification is achieved by the use of a thermo stable DNA polymerase (isolated from a bacterium, Thermusaquaticus), which remain active and stable during the high temperature and induceddenaturation of double-standard DNA.

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