11th Class Biology Tools And Techniques Cytochemistry

Cytochemistry

Category : 11th Class

A number of dyes or stains are known to colour specific parts. Certain dyes can be used even in case of living materials. They are called vital stains, e.g., neutral red, methylene blue.

Fuelgen or Schiff?s reaction was developed by Fuelgen and Rossenbeck (1924). Identification and localization of chemical compounds  of a cell is studies in cytochemistry.

Some important cytochemical stains

Stain

Used for staining

Final colour

Acetocarmine

Chromosomes

Pink

Acid fuchsin

Cortex, cellular walls, mitochondria

Magenta

Aniline blue

Fungal hyphae

Blue

Basic fuchsin

Nucleus

Magenta red

Crystal violet

Bacteria

Violet

Eosin

Cytoplasm

Pink

Feulgen's stain

DNA

Purple/Red

Haematoxyline

Nuclei, cell wall, cellulose

Violet

Iodine solution

Starch

Blue

Janus green

Fungi and mitochondria

Green

Methylene blue

Yeast and Golgi complex

Blue

Phloroglucinol +HCl

Lignin

Red

Ruthenium red

Pectin

Red

Saffranin

Nuclei, lignified tissue

Red

Sudan- III or IV

Suberin, cutin, oil

Red

Sudan black

Fatty substance

Black

Toludine blue

RNA

Blue

Cotton blue

Fungi

Violet



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