Category : 11th Class
A number of dyes or stains are known to colour specific parts. Certain dyes can be used even in case of living materials. They are called vital stains, e.g., neutral red, methylene blue.
Fuelgen or Schiff?s reaction was developed by Fuelgen and Rossenbeck (1924). Identification and localization of chemical compounds of a cell is studies in cytochemistry.
Some important cytochemical stains
|
Stain |
Used for staining |
Final colour |
|
Acetocarmine |
Chromosomes |
Pink |
|
Acid fuchsin |
Cortex, cellular walls, mitochondria |
Magenta |
|
Aniline blue |
Fungal hyphae |
Blue |
|
Basic fuchsin |
Nucleus |
Magenta red |
|
Crystal violet |
Bacteria |
Violet |
|
Eosin |
Cytoplasm |
Pink |
|
Feulgen's stain |
DNA |
Purple/Red |
|
Haematoxyline |
Nuclei, cell wall, cellulose |
Violet |
|
Iodine solution |
Starch |
Blue |
|
Janus green |
Fungi and mitochondria |
Green |
|
Methylene blue |
Yeast and Golgi complex |
Blue |
|
Phloroglucinol +HCl |
Lignin |
Red |
|
Ruthenium red |
Pectin |
Red |
|
Saffranin |
Nuclei, lignified tissue |
Red |
|
Sudan- III or IV |
Suberin, cutin, oil |
Red |
|
Sudan black |
Fatty substance |
Black |
|
Toludine blue |
RNA |
Blue |
|
Cotton blue |
Fungi |
Violet |
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