Pharmacy

Bio-Rad Laboratories, Inc's new EpiQ™ Chromatin analysis kit is a real-time PCR assay for the rapid quantitative assessment of chromatin  structure. Complementing existing epigenetic assays is DNA methylation and chromatin

Immuneprecipititation, the EpiQ™ kit Is The first commercial research tool helps scientists quantify the impact: of epigenetic events on gene expression regulation through chromatin State changes.

The EpiQ™ kit can provide alarsritative information about chromatin accessibility, which correlates strongly with gene expression. The key benefits of the EpiQ™ chromatin analysis kit include assessment of chromatin structure in cultured cells within six hours, requirement of relatively few cells (as few as 50,000) to perform analysis and generation of quantitative chromatin structure information for multiple genomic elements.

The chromatin state of a gene can be identified in situ based on its sensitivity to the action of the nuclease in the EpiQ™ kit. In heterochromatin, genomic DNA is inaccessible to nuclease digestion and remains available for subsequent qPCR. Analysis of heterochromatin using the EpiQ™ kit reveals a minimal quantification cycle (Cq) shift between digested and undigested samples.